By Ashok Agarwal, Sajal Gupta, Rakesh Sharma
This cutting-edge laboratory handbook comprises 20 medical protocols used day-by-day for the research of the infertile male, awarded with effortless to appreciate, step by step method. The protocols are prepared from regimen to complex laboratory strategies universal to scientific perform, together with computer-assisted semen research, sperm education for IUI by means of density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation attempt through TUNEL procedure, between others. The method in every one protocol follows most sensible perform directions made clearer via professionally hand-drawn illustrations protecting lots of the very important steps and kit. The authors, hailing from the world-renowned Andrology middle at Cleveland health center, have over 50 years of mixed first-hand adventure in dealing with very busy diagnostic and study amenities in male infertility and andrology. The publication might be an fundamental source for hundreds of thousands of laboratory technologists, clinicians and reproductive execs (andrologists, embryologist, etc.) engaged within the prognosis and administration of infertile males round the world.
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Additional info for Andrological Evaluation of Male Infertility: A Laboratory Guide
4. Mortimer D, Mortimer ST. Quality and risk management in the IVF laboratory. Cambridge: Cambridge University Press; 2005. 5. World Health Organization (WHO). Chapter 7. Quality assurance and quality control. In: WHO laboratory manual for the examination and processing of human semen. Geneva: World Health Organization; 2010. 6. Björndahl L, Mortimer D, Barratt CLR, Castilla JA, Menkveld R, Kvist U, Alvarez J, Haugen TB. Chapter 10. Quality management and accreditation. In: A practical guide to basic laboratory andrology.
A semen sample known to be free of ASA must be used for preparing controls (or the negative control will fail). Discarded or donated semen can be cryopreserved for this purpose. , difference between technicians) should be routinely determined by side-by-side testing as discussed in the next chapter. This must be documented and can be used to detect when retraining is required. Interlaboratory evaluation or proﬁciency testing is mandated by some governments, including the Clinical Laboratory Improvement Act (CLIA) in the USA.
Some disposable chambers differ signiﬁcantly from hemocytometry, and this must be 2 Quality Management in Andrology Laboratory 21 Fig. 1 Histograms showing the differences in bead counts between the upper and lower rows of a single Makler chamber. These counts were made over 495 days of testing. (a) High bead concentrations. (b) Low bead concentrations taken into account when calculating and reporting results . Swan et al.  found that MicroCell chambers produced sperm concentration values that were signiﬁcantly lower than those for hemocytometry, and some microbead control products give separate determined values for the hemocytometer and MicroCell chambers.
Andrological Evaluation of Male Infertility: A Laboratory Guide by Ashok Agarwal, Sajal Gupta, Rakesh Sharma